Peer-Reviewed Publications

Buckley M.*^, Jacob W.P.*^, Bortey L.*, McClain M.E.*, Ritter A.L.*, Godfrey A.*, Munneke, A.*, Ramachandran, S., Kenis, S., Kolnik, J.*, Olofsson, S., Nenadovich, M.*, Kutoloski, T.*, Rademacher, L.*, Alva, A.*, Heinecke, O., Adkins, R.*, Parkar, S.*, Bhagat, R.*, Lunato, J.*, Beets, I., Francis, M.M., Kowalski, J.R. 2024. Cell non-autonomous signaling through the conserved C. elegans glycoprotein hormone receptor FSHR-1 regulates cholinergic neurotransmission. PLoS Genet 20(11): e1011461. https://doi.org/10.1371/journal.pgen.1011461. PMID: 39561202; PMCID: PMC11614273. *Undergraduate co-author. ^ Equal contributions.

Micropublication: Rana M.^, Kowalski J. oxi-1 is required for chemotaxis to odorants sensed by AWA but not AWC neurons. MicroPubl Biol. 2024 Aug 19;2024:10.17912/micropub.biology.001282. doi: 10.17912/micropub.biology.001282. PMID: 39228993; PMCID: PMC11369694. ^High school co-author.

Micropublication: Hulsey-Vincent, H; Alvinez, N; Witus, S; Kowalski, JR; Dahlberg, C. 2023. A Fiji process for quantifying fluorescent puncta in linear cellular structures. microPublication Biology. 10.17912/micropub.biology.001003.

Micropublication: Hulsey-Vincent, H; Athanasopoulos, A; McGehee, A; Kowalski, JR; Dahlberg, C. 2023. A Fiji protocol for analyzing puncta is a robust tool for measuring GLR-1::GFP accumulation in the ventral nerve cord of C. elegans. microPublication Biology. 10.17912/micropub.biology.001004.

Micropublication: Hulsey-Vincent, H; McClain, M*; Buckley, M*; Kowalski, JR; Dahlberg, CL. 2023. Comparison and agreement between two image analysis tools for quantifying GFP::SNB-1 puncta in fshr-1 mutants of C. elegans. microPublication Biology. 10.17912/micropub.biology.001005. *Undergraduate co-author.

Kowalski, J.R., Lineweaver, T., Novak, K.B. 2022. Developing Integrative Thinking in Undergraduate Students through an Interdisciplinary General Education Course on Mental Illness. College Teaching, 70:4, 493-505, DOI: 10.1080/87567555.2021.1982856.

Kreyden, V.,* Mawi, E.*, Rush, K.M.*, Kowalski, J.R. 2020. UBC-9 Acts in GABA Neurons to Control Neuromuscular Signaling in C. elegans. Neurosci Insights 2020 Oct 5;15:2633105520962792. doi: 10.1177/2633105520962792. PMID: 33089216; PMCID: PMC7543134.*Undergraduate co-author.

Micropublication:  Wei, B.^, Kowalski, J.R. (2018). oxi-1 and fshr-1 are required for neuromuscular signaling under normal and  oxidative stress conditions in C. elegans. MICROPUBLICATION:BIOLOGY https://doi.org/10.17912/PFYW-FT85.
^High school co-author.

Dube, S.*, Dube, H.*, Green, N.B.*, Larsen, E.M., White, A.*, Johnson, R.J. and Kowalski, J.R. (2017). In Vivo Delivery and Activation of Masked Fluorogenic Hydrolase Substrates by Endogenous Hydrolases in C. elegans. ChemBioChem. doi:10.1002/cbic.201700278.
*Undergraduate co-author.

Deffit, S.N., Neff, C., and Kowalski, J.R. (2017). Exploring C. elegans behavior: An inquiry-based laboratory module for middle or high school students. Am Biol Teach, 79:661-667.

Kowalski J.R., Hoops, G., and Johnson, R.J. (2016). Implementation of a Collaborative Series of Classroom-Based Undergraduate Research Experiences Spanning Chemical Biology, Biochemistry, and Neurobiology. CBE Life Sci Educ, 15: ar55. DOI:10.1187/cbe.16-02-0089.

Wang, J.^, Jennings, A.K., and Kowalski, J.R. (2016). The Anaphase-Promoting Complex (APC) ubiquitin ligase affects chemosensory behavior in C. elegans. PeerJ, 4:e2013 https://doi.org/10.7717/peerj.2013.
^High school co-author.

Kowalski, J.R., Dube, H.*, Touroutine, D., Rush, K.M.*, Goodwin, P.R., Carozza, M., Didier, Z.*, Francis, M.M., and Juo, P. (2014). The Anaphase-Promoting Complex ubiquitin ligase regulates GABA transmission at the C. elegans neuromuscular junction. Mol Cell Neurosci, 58:62-75.
*Undergraduate co-author.

Kowalski, J.R. and Juo, P. (2012).  The role of deubiquitinating enzymes in synaptic function and nervous system diseases. Neural Plasticity, 2012: 13 pages. Article ID: 892749, doi:10.1155/2012/892749.
Review article.

Monteiro M.I., Ahlawat S., Kowalski J.R., Malkin E., Koushika S.P., Juo P. (2012). The kinesin-3 family motor KLP-4 regulates anterograde trafficking of GLR-1 glutamate receptors in the ventral nerve cord of Caenorhabditis elegans. Mol Biol Cell, 23:3647-62.

Kowalski, J.R., Dahlberg, C.L., and Juo, P. (2011). The deubiquitinating enzyme USP-46 negatively regulates the degradation of glutamate receptors to control their abundance in the ventral nerve cord of C. elegans. J. Neuroscience, 31: 1341-1354.

Yang, L., Kowalski, J.R., Yacono, P., Bajmoczi, M., Shaw, S.K., Froio, R.M., Golan, D.E., Thomas, S.M. and Luscinskas, F.W.  (2006). Endothelial cell cortactin coordinates ICAM-1 clustering and actin cytoskeleton remodeling during polymorphonuclear leukocyte adhesion and transmigration.  J. Immunology, 177:6440-6449.

Yang, L., Kowalski, J.R., Zhan, X., Thomas, S.M., and Luscinskas, F.W.  (2006). Endothelial cell cortactin phosphorylation by Src contributes to polymorphonuclear leukocyte transmigration in vitro.  Circ. Res. 98:394-402.

Kowalski, J.R., Egile, C., Gil, S., Snapper, S.B., Li, R., and Thomas, S.M.  (2005). Cortactin regulates cell migration via activation of N-WASP.  J. Cell Sci. 118:79-87.

Hastings, P.J., Bull, H.J., Klump, J.R., and Rosenberg, S.M.  (2000) Adaptive amplification, an inducible chromosomal instability mechanism.  Cell 103:723-731.

External Grants

NIH R15 (AREA) award (2022-2025), $ 422,267, National Institutes of Health, “Investigation of the G protein - coupled receptro FSHR-1 in multi-tissue neuromuscular signaling in normal and oxidative stress conditions”.

NSF MRI grant, co-PI (2021-2024), $472,138, National Science Foundation, “MRI: Acquisition of Spinning Disk Confocal for Multi-Disciplinary Research and Undergraduate Teaching and Training”. (w/ PI Lindsay Lewellyn, co-PIs Jeremy Johnson, Chris Stobart, Patience Masamha, and major users Mark Macbeth and Conrad Hong).

Senior Research Award (2017-2018), Indiana Academy of Sciences, $2,500,“Investigation of neuromuscular signaling control by the SUMO conjugating enzyme, UBC-9”.

Visiting Scientist Fellowship (2015), Mount Desert Island Biological Laboratory. $400 plus lab space and fees. “Generation of transgenics via CRISPR-Cas9 in C. elegans.”

Senior Research Award (2014-2015), Indiana Academy of Sciences, $3,000,“Characterization of the neuronal functions of SUMO enzymes regulating neuromuscular signaling in C. elegans”.

ASCB-COMPASS Outreach award, co-PI (2015), $830. “Middle School Neurobiology Inquiry-based Lab Module” (co-investigator with Dr. Sarah Deffit, Indiana University)

NIH R15 (AREA) award (2012-2017), $372,869, “Investigation of  Anaphase Promoting Complex function  in regulating synaptic transmission”.

NSF TUES award, co-PI with R.J. Johnson and G. Hoops (Chemistry), (2012-2016), $199,942, “An integrated series of student-driven, research-based undergraduate laboratory courses linking chemical biology, biochemistry, and neurobiology”.

Senior Research Award (2011-2012), Indiana Academy of Sciences, $2,843, “Investigation of SUMO enzyme function in regulating synaptic transmission at the C. elegans neuromuscular junction”

SENCER Post-Institute Implementation award (2010), $2,705 shared award with other members of the Butler 2010 SENCER Team.   NW course proposal with Angela Ockerman (COPHS): “Life, Death and Immortality:  Henrietta Lacks and the HeLa revolution”.

Cottrell College Science Award (2010-2012), Research Corporation, $35,000 “Identification and characterization of Anaphase Promoting Complex substrates that regulate synaptic transmission in C. elegans”

Training in Education and Critical Research Skills (TEACRS) Postdoctoral Fellowship (2006-2009), NIH/National Institute of General Medical Science (NIGMS), IRACDA program

Graduate Research Fellowship (2002-2005), National Science Foundation